glucose가 종자의 발아와 생장에 미치는 영향

glucose가 종자의 발아와 생장에 미치는 영향

glucose가 종자의 발아와 생장에 미치는 영향.pptx

목차 1. Object 2. Introduction 3. Methods & Material 4. Results 5. Discussion & conclusion 6. Reference 본문 1. Objects To understand Plant stress responses To find glucose responses on plant germination and growth. To understand plant glucose and hormonal interplay 2. Introduction ▶ Plant stress External conditions that adversely affect growth, development, or productivity Stresses trigger a wide range of plant responses: altered gene expression cellular metabolism changes in growth rates and crop yields ▶ Plant stress factors Biotic - imposed by other organisms Abiotic - arising from an excess or deficit in the physical or chemical environment -> Plant responses to abiotic factors : arising from an excess or deficit in the physical or chemical environment -> Excess exogenous Glucose is also a environmental cue! ▶ Glucose treatment : 3%, 4%, 5% Glc medium ** 전부 clean bench에서 무균작업으로 수행할 것 준비한 종자를 적정량 1.5ml tube에 담고 70% Ethanol 200ul씩 3회 washing 2% NaOCl 200ul 10분 treatment 3차증류수 200ul 5회 washing Washing 후 다시 3차 증류수 200ul을 넣은 뒤 pipetting으로 종자를 배지 위에 물기가 없도록 올려놓는다. 2주간 관찰, 발아율, 뿌리길이, cotyledon 활착 여부, 엽색의 변화 등을 관찰 참고문헌 Rognoni, S., Teng, S., Arru, L., Smeekens, S. C., & Perata, P. (2007). Sugar effects on early seedling development in Arabidopsis. Plant Growth Regulation, 52(3), 217-228. Sheen, J., Zhou, L., & Jang, J. C. (1999). Sugars as signaling molecules. Current opinion in plant biology, 2(5), 410-418. Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and environmental safety, 60(3), 324-349. Arenas-Huertero, F., Arroyo, A., Zhou, L., Sheen, J., & León, P. (2000). Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes & Development, 14(16), 2085-2096. Li, Y., Lee, K. K., Walsh, S., Smith, C., Hadingham, S., Sorefan, K., . & Bevan, M. W. (2006). Establishing glucose-and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine. Genome Research, 16(3), 414-427. Xiao, W., Sheen, J., & Jang, J. C. (2000). The role of hexokinase in plant sugar signal transduction and growth and development. Plant molecular biology, 44(4), 451-461. http://en.wikipedia.org/wiki/Abscisic_acid http://en.wikipedia.org/wiki/Ethylene#Ethylene_as_a_plant_hormone 키워드 발아, 종자